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In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus.pdf
Hindawi Publishing Corporation
Journal of Parasitology Research
Volume 2009, Article ID 624919, 7 pages
doi:10.1155/2009/624919
Research Article
In Vitro Study of Nitric Oxide Metabolites Effects on Human
Hydatid of Echinococcus granulosus
Razika Zeghir-Bouteldja, Manel Amri, Saliha Aitaissa, Samia Bouaziz,
Dalila Mezioug, and Chafia Touil-Boukoffa
Laboratoire de Biologie Cellulaire et Moleculaire, FSB-USTHB, Universite Bab-Ezzouar, Bp 32, 16111 Algiers, Algeria
´ ´
Correspondence should be addressed to Chafia Touil-Boukoffa, touilboukoffa@yahoo.fr
Received 30 June 2009; Accepted 4 September 2009
Recommended by Claudio Genchi
Hydatidosis is characterized by the long-term coexistence of larva Echinococcus granulosus and its host without effective rejection.
Previous studies demonstrated nitric oxide (NO) production (in vivo and in vitro) during hydatidosis. In this study, we investigated
the direct in vitro effects of NO species: nitrite (NO− ), nitrate (NO− ) and peroxynitrite (ONOO− ) on protoscolices (PSCs) viability
2 3
and hydatid cyst layers integrity for 24 hours and 48 hours. Our results showed protoscolicidal activity of NO− and ONOO−
2
24 hours and 3 hours after treatment with 320 μM and 80 μM respectively. Degenerative effects were observed on germinal and
laminated layers. The comparison of the in vitro effects of NO species on the PSCs viability indicated that ONOO− is more
cytotoxic than NO− . In contrast, NO− has no effect. These results suggest possible involveme
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