extraction of flavonoids from garden (salvia officinalis stronglstrong.).pdfVIP

J. Serb. Chem. Soc. 72 (1) 73–80 (2007) UDC 635.74+664. 1.035:547.972.2/.3+547.962.6 JSCS–3536 Original scientific paper Extraction of flavonoids from garden (Salvia officinalis L.) and glutinous (Salvia glutinosa L.) sage by ultrasonic and classical maceration 1* 2 2 DRAGAN T. VELI^KOVI] , MILENA T. NIKOLOVA , STEPHANIE V. IVANCHEVA , JELENA B. STOJANOVI]1 and VLADA B. VELJKOVI]3# 1Zdravlje-Actavis Company, 199 Vlajkova St., 16000 Leskovac, 2 Institute of Botany, Bulgarian Academy of Sciences, 23 G. Bunchev St., 1113 Sofia, Bulgaria and 3Faculty of Technology, 124 Bulevar oslobo|enja St., 16000 Leskovac, Serbia (e-mail: dvelickovic@actavis.co.yu) (Received 2 December 2005, revised 10 May 2006) Abstract: Flavonoids were analysed in the extracts of garden (Salvia officinalis L.) and glutinous (Salvia glutinosa L.) sage. Ultrasonic extraction (20 minutes at 40 °C) and clas- sical maceration (6 h at room temperature) of the extractable substances from dried herbs and dried residual plant materials from which the essential oil had previously been re- moved by hydrodistillation were performed with petroleum ether, 70 % aqueous solution of ethanol and water. It was found that the extracts from both plants contained flavonoids, but their compositions were dependent of the plant species, the polarity of the extracting solvent and the extraction technique applied. Apigenin and its derivatives (e.g., apigenin 4-methyl ether), scutellarein 6-methyl ether, isoscutellarein 8-methyl ether, luteolin and 6-OH-luteolin-6-methyl ether where distinctive for