分离筛选具有抑活性的海洋放线菌.pdf

Isolationand ofmarine antibacterial screening Actinomycesproducing substances Abstract objective： 1 Toisolateandscreenantibacterial fromthe itsbiochemical Actinomycesocean，analyze and16SrDNAand thestrain’S properties identify species． 2 Tomakea onthefactors antibacterialof priliminaryinvestigation influencing activity fermentation thefermentationconditions． supernatant，optimize Materialsandmethods： as turbidsolutions 1 Seamudscollectedfromthe coast dilution，the Qingdao samples，aftergradient totheGause’Sculture dichromateconcentration50p werecoated plate(potassium g／mL、to isolatemarine aureusandEscherichiacoliasthe Actinomycete．Staphylococcus indicating madea ontheantibacterialof bateria,we activity pathogenic preliminaryinvestigation fermentation thecircularmethod． by slip supernanants 2 Tothe withantibacterial and Actinomycetes activity,morphological biochemical 6SrDNA and treewereconducted． identification，1 properties analysisphylogenic 3 fermentation theinfluencefermentationtimeonthe Keeping tempretureconstant，study time theinfluencefermentmion antibacterial fermentation activity．；Keeping constant，study