实验6兔血清中茶碱的高效液相色谱法测定解决方案.pptVIP

Pharmaceutical Analysis 2012-11 Experiment Ⅵ Determination of theophylline in rabbit plasma by HPLC 1. Purpose (1) To learn the serum sample treatment method and to be familiar with the blood sample collection method (2) To learn the experiment on the determination of theophylline in rabbit serun by HPLC 2. Introduction Theophylline Caffine N O N H3C—N N O CH3 H 3. Procedure and Method (1) Preparation of reference standards solution Theophylline （THP）standard solution: 1.0 mg/mL (2) Preparation of internal standard solution Caffe