惰性载体对瘤胃真菌产酶活性的影响设计.docVIP

青岛农业大学 毕 业 论 文 题 目：惰性载体对瘤胃真菌产酶活性的影响 姓 名： 学 院： 动物科技学院 专 业： 动物科学 班 级： 1001 学 号： 指导教师： 2014 年 6 月 5 日 惰性载体对瘤胃真菌产酶活性的影响 动物科学专业 指导教师 王利华 本试验采用二因素试验设计，通过体外固态发酵试验，利用从崂山奶山羊瘤胃内分离纯化的一株真菌菌株探讨不同惰性载体（珍珠岩、沸石、膨润土）及不同添加量（0.2%、0.4%、0.6%）对瘤胃真菌产羧甲基纤维素酶、β-葡萄糖苷酶、微晶纤维素酶、滤纸酶酶活的影响。结果表明，沸石、膨润土添加组显著提高羧甲基纤维素酶活（P0.05），载体添加量对酶活影响不显著（P0.05）。载体类型、添加量及载体类型与添加量互作对对微晶纤维素酶、β-葡萄糖苷酶和滤纸酶均有显著影响（P0.05），以沸石为载体、添加量为0.2%时产酶活性最高。在发酵第8天时，各组酶活较高。综上所述，本试验条件下载体为沸石、添加量为0.2%、培养8天对瘤胃真菌产纤维素酶活高。 Influence of inert Carrier on Cellulase Activities fermented by Ruminal Fungus Student Majoring in Animal Science Wu Shuowen Tutor Wang Lihua Abstract: Solid-state fermentation in vitro was adopted to study the effects of inert carrier types and supplements on cellulose activities by two factors experimental design. Carrier types included perlite, zeolite, bentonite, and carrier supplements were 0.2%, 0.4% and 0.6%, respectively. A fungus strain purified and isolated from the rumen of Laoshan dairy goats was fermented to produce cellulases. Carboxymethyl cellulase, β-glucosidase, microcrystalline cellulase and filter paper enzyme were monitored. Compared to bentonite, zeolite and bentonite significantly improved Carboxymethyl cellulase (P0.05), and the carrier supplements had no significant effects on the Carboxymethyl cellulase (P0.05). Carrier types, carrier supplements and the interaction of carrier types with supplements had significant effects on β-glucosidase, microcrystalline cellulase and filter paper enzyme (P0.05). The zeolite supplement of 0.2% had the greatest activity of β-glucosidase, microcrystalline cellulase and filter paper enzyme. The cellulase activities had the greatest at fermentation time point of 8 day. It could be concluded from results tha