VitaminD疾病和实验室检测-曹博士要点.pptVIP

* 维生素D使用过量 – 儿童中毒 2岁男孩送至急诊，便秘，呕吐和腹痛， 3天， 体征：血压轻度升高，其它：良好 出院诊断：便秘 第二天，因腹痛加重送回急诊 母亲给他4天每天1安瓿（600,000 IU）VD，即男孩在4天里一共使用了2,400,000 IU PEDIATRICS Vol. 116 No. 3 September 2005, pp. e453-e456 * 标准样品SRM972的实用性使方法标准化成为可能 * SRM972的成分 * SRM972的色谱学分析 洗脱时间（分） 洗脱时间（分） 洗脱时间（分） 洗脱时间（分） 强度（cps） 强度（cps） 强度（cps） 强度（cps） * 总结 维生素D缺乏与众多医疗情况有关 补充维生素D可以预防某些机体功能紊乱 过度使用维生素D引起中毒 实验室25-羟基-维生素D检测作为体内维生素D状态的一种指标 * 总结 – 续 免疫分析法和HPLC-串联质谱测量法被大多数实验室用来测量25-羟基-维生素D 这些方法缺乏标准化，因此结果存在差异 原因是参考范围的确定缺乏一致的标准 诊断维生素D缺乏或不足需要考虑检测方法和合适的参考范围 * John Bennett Fenn (born June 15, 1917, New York City) is an American research professor of analytical chemistry who was awarded a share of the Nobel Prize in Chemistry in 2002. Fenn won the award for his work in the field of mass spectrometry, specifically for the electrospray ionization technique often used to identify and analyze biological macromolecules. He was awarded the Association of Biomolecular Resource Facilities Award for outstanding contributions to Biomolecular Technologies in 2002. Fenns discovery quickly produced broad practical benefits. For example, it rapidly increased the speed with which complex new pharmaceutical compounds could be evaluated, leading directly to the development of life-saving AIDS medications (protease inhibitors) in the mid-1990s. Fenn, born in New York City to parents who were living in Hackensack, New Jersey, later moved with his family to Berea, Kentucky as a young teenager. He earned an A.B. from Berea College in his new hometown and a Ph.D. from Yale University in 1940. He then spent three years at Princeton University as Director of Project SQUID, a funded by the Office of Naval Research. “(Willard Gibbs) The greatest thermodynamicist of them all”—John Fenn, Engines, Energy, and Entropy[1]He joined the Yale University faculty in 1962. In 1987, he reached the mandatory retirement age. Fighting age discrimination and a University-mandated move to smaller laboratory space, Fenn remained at Yale and was 70 years old befor