不同给氢方式对家兔氢气代谢的影响.doc

腹腔氢气与腹腔氢水注射对大鼠氢气代谢的比较 林涛1 黄国庆2 文明祥2 廖晓星2( （1. 桂林市第二人民医院541001 2.中山大学附属第一医院急诊科，广州 510080） 摘要 目的：比较腹腔注射氢气与氢水对鼠氢气代谢的影响。方法：20只SD大鼠，在监测呼气氢气基础值后，均先后予以氢气及饱和氢气生理盐水1ml/100g及2ml/100g腹腔注射。记录不同给氢方式呼气氢气浓度的达峰时间，峰浓度，计算半衰期和曲线下面积，比较不同给氢方式对呼气氢气浓度的影响。 结果：腹腔注射1ml/100g氢水、氢气达峰时间（Tmax）分别为3.48±1.54min、15.85±7.24min，注射2ml/100g氢水、氢气分别为5.48±1.88min、21.25±11.65min，同剂量不同方式比较及同方式不同剂量比较均差异显著（P0.01）；单倍剂量氢水、氢气峰浓度（Cmax）分别为35.04±12.43ppm、44.43±16.13ppm，双倍剂量氢水、氢气分别为49.45±18.89ppm、62.82±23.74ppm，同剂量不同方式比较及同方式不同剂量比较均差异显著（P0.01）；单倍剂量氢水、氢气半衰期（T1/2）分别为8.37±3.22min、125.08±73.68min，双倍剂量氢水、氢气分别为11.21±4.84min、168.46±85.77min，同剂量不同方式比较及同方式不同剂量比较均差异显著（P0.01）；单倍剂量氢水、氢气较基础值增加的曲线下面积（AUC）分别为279.43±161.67ppm·min、4249.87±1689.74 ppm·min，双倍剂量氢水、氢气分别为384.03±172.25ppm·min、6834.04±2471.67ppm·min，同剂量不同方式比较及同方式不同剂量比较均差异显著（P0.01）。 结论：腹腔注射氢水、氢气均可迅速提高呼气氢气浓度，存在量效关系，腹腔注射氢气能长时间维持呼气氢气高浓度。 关键词 氢气；饱和氢气生理盐水；大鼠；代谢 Hydrogen metabolism in rabbits by different ways of hydrogen supplied LIN Tao1, HUANG Guo-qing2, WEN Ming-xiang2, LIAO Xiao-xing2* （1. Department of Emergency, The Second Peoples Hospital of Guilin, Guilin 541001,China; 2.Department of Emergency, The First Affiliated Hospital, Sun Yat-Sen University, Guanghou 510080, China; ） To comparison the effect of intraperitoneal injection saturated hydrogen saline or hydrogen gas on hydrogen metabolism in rats. Methods: Twenty SD rats were tested the basis concentration of exhaled hydrogen. Then all of them were supplied saturated hydrogen saline or hydrogen gas at 1ml/100g and 2ml/100g intraperitoneal respectively. Exhaled hydrogen concentration were monitored continuously. Record the peak time of eshaled hydrogen concentration（Tmax）, the max eshaled hydrogen concentration（Cmax）, calculated half-life （T1/2） and area under the curve（AUC）. Then comparing different ways to the effect of exhaled hydrogen concentration. Results: When intraperitoneal saturated hydrogen saline or hydrogen at 1ml/100g, the Tmax were 3.48 ± 1.54min vs 15.85 ± 7.24min, and the Tmax were 5.48 ± 1.88min vs