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桁架强度及稳定性计算 模板强度验算: 已知条件: 已知桁架的尺寸(如图1-1),宽800mm,长5000mm?上?下弦杆截面选用16#工字钢, b=88mm,A=26.1,=6.58cm?截面高度h=800mm?缀板中距为800mm?竖缀板为16#工字钢,斜缀板为10#槽钢?混凝土浇注速度:1m/h ;浇注温度5—30℃,取15℃计算;初凝时间:8h;塌落度:16—18cm;添加高性能混凝土外加剂.风荷载按最大风力8级考虑 图a(一)?荷载 新浇混凝土侧压力标准值最大值F= —混凝土密度 ( ) (8h) 外加剂影响修正系数,不参外加剂时取1.0,参高性能混凝土外加剂时取1.2混凝土塌落度影响修正值,塌落度180mm时取1.3 F===68.64 KN/㎡新浇混凝土侧压力设计值F1=68.64X1.2=82.36 KN/㎡倾倒混凝土时荷载标准值,4 KN/㎡,其设计值值F2=4X1.4=5.6 KN/㎡=F1+F2=82.36+5.6=88KN/㎡(二)桁架参数分析:荷载 桁架节点荷载设计值:P=qa =0.088×800×800=56.32KN内力计算:上弦杆:腹杆:二强度计算:(一)上弦杆弯矩: 用16#工字钢, 轻型钢结构设计手册中(3.4-27)计算的稳定性为: (二)腹杆:,选10#槽钢: 轻型钢结构设计手册中(3.4-27)计算的稳定性为: 故桁架满足要求(三)挠度验算:板的最大挠度:16#工字钢:10#槽钢: 故满足要求 说明:此验算过程中引用的公式和参数来自建筑工程模板施工手册 中国建筑工业出版社《轻型钢结构设计手册》 中国建筑工业出版社 2009年1月3日Acknowledgments The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC 103-2321-B-400-008). Author Contributions Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and S.J.L. wrote the manuscript. Additional Information C57BL/6 mice were immunized subcutaneously (s.c.) once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were removed, and a biotinylated anti-IFN-γ Ab (eBioscience, San Diego, CA, USA) was added to each well. The plates were incubated at 37 °C for 2 h. Following the addition of the avidin