醋酸钙不动杆菌的分离鉴定及其溶藻特性.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 3 :140-145 醋酸钙不动杆菌的分离鉴定及溶藻特性 赟   王   张业猛  李佩佩 （河南城建学院生命科学与工程学院，平顶山 467036） 摘 要 ： 淡水微囊藻水华不仅造成水体动植物缺氧死亡，而且释放藻毒素，影响人类和其它动物的健康。利用液体感染技术， 从河南省平顶山市白龟山水库分离一株能够溶解铜绿微囊藻PCC 7806 的溶藻菌，命名为溶藻菌5，16S rDNA 核苷酸序列测序证实 该菌株为醋酸钙不动杆菌。它具有一定的溶藻特异性，只溶解PCC 7806，对FACHB-930 和斜生栅藻没有影响，能够促进衣藻和红 球藻的生长。最佳溶藻体积比为1 ∶1。溶藻菌5 的菌体和无细胞培养物均具有相同的溶藻效果。显微观察藻细胞被溶解的黄化液 显示细菌并未附着在藻细胞周围，也无菌胶膜形成。表明溶藻菌5 可能通过释放杀藻物质和与藻竞争营养物质两种机制溶解藻细胞。 关键词 ： 溶藻菌 ；醋酸钙不动杆菌 ；16S rDNA ；铜绿微囊藻 ；溶藻特性 DOI ：10.13560/ki.biotech.bull 1985.2015.04.020 ， Isolation Identification and Algicidal Activity of Acinetobacter calcoaceticus Wang Yun Zhang Yemeng Li Peipei （College of Life and Engineering ，Henan University of Urban Construction ，Pingdingshan 467036 ） Abstract: The microcystis bloom not only result in the death of the aquatic animals due to hypoxia, but also produce mycrocystins which is harmful to the human beings and wild animals. One algicidal bacterium against toxic Microcystis aeruginosa PCC 7806 was isolated from the Baiguishan Reservoir in Pingdingshan of Henan province using liquid infection technology and was named algicidal bacterium 5. The 16S rDNA sequence analysis indicated that this bacterium belongs to Acinetobacter calcoaceticus . It specifically removes PCC 7806 by cell lysis, but has no effect on FACHB-930 and Scenedesmus obliquus. Interestingly, the growth of haematococcus and Chlamydomonas reinhardtii are promoted. The algicidal effect of bacterium against PCC 7806 is better when the volume ratio of 1∶1. The thal