人参皂苷Rh2对荷肺癌小鼠基因表达的肿瘤特异性调节.docVIP

人参皂苷Rh2 对荷肺癌小鼠基因表达的肿瘤特异性调节 张文静,包丽华,俞春莺,张军毅,赵　安,许长江,贾韦国 Tumor Specif ic Gene Regulation by Ginseng Saponin Rh2 in Mice Bearing Lung Cancer ZHANG Wen2jing , BAO Li2hua , YU Chun2ying , ZHANG J un2yi , ZHAO An , XU Chang2jiang , J IAWei2guo S hanghai Innovati ve Research Center of TCM , S hanghai 201203 , China Corres ponding A uthor : J IA Wei2guo , E2mai l :w j ia@interchange. ubc. ca Abstract :Objective 　To study gene differential expression profiles by ginsenoside Rh2 in normal and tumor2bearing mice. Methods 　Gene expression profiles af ter t reatment with Rh2 in normal and tumor2bearing mice were determined by Oligo chip technology as well as the clustering and statistic analysis of single gene. To further validate the microarray result s ,we used quantitative real2time RT2PCR to examine the effect of Rh2 on individual genes. Results 　As compared with normal mice , t reatment with Rh2 changed gene expression more potently in mice bearing tumor. Thereps no obvious correlation on the gene expression profiles between the normal and tumor2bearing mice. Treatment with Rh2 was found to be ef2 fectively resistant abnormal gene expression profiles in some major organs that resulted f rom the local im2 plantation of tumor. Conclusion 　The data showed that there is specific regulation of gene expression pro2 files related to tumor by Rh2 in mice bearing tumor. Key words :Rh2 ; Oligo chip assay ; Gene expression profile 摘　要:目的　研究人参皂苷Rh2 对正常和荷肺癌小鼠基因表达的调节差异。 方法　运用寡核苷酸芯片技术,检测Rh2 对正常和荷瘤小鼠的基因表达调节,并对差异基因进行聚类和统计分析。为了进一步验证芯片结果,我们使用实时荧光定量PCR 方法检验Rh2 对基因表达的作用效果。 结果　与正常小鼠相比,Rh2 对荷瘤小鼠的基因表达影响更大,二者的主要受调脏器也不同;Rh2 对正常和肿瘤小鼠基因表达调节没有明显的相关性;Rh2 能有效拮抗局部接种肿瘤引起的荷瘤小鼠各脏器基因异常表达。 结论　Rh2 对荷瘤小鼠的基因表达调节具有肿瘤特异性。 关键词:人参皂苷Rh2 ;基因芯片;基因表达谱 中图分类号:R734. 2 ;Q344 + . 13 　　文献标识码:A 　　文章编号:100028578 (2008) 0820555205 0 　引言 人参(Panax ginseng C. A. meyer) 为五加科多年生草本植物,是有几千年应用历史的名贵中药。其效果不仅早为古今中外无数医学家肯定,而且为现代科学研究所证明。最早对人参药理功用给予高度评价的,首推《神农本草经》。书中明确指出,人参“主补五脏,安精神,止惊悸,除邪气,明目,开心益智”。 人参主要含有皂苷、人参多糖、多肽类、氨基酸及维