Identification of stem cells of cervical cancer.pptVIP

Identification of stem cells of cervical cancer Zhu ping China Three Gorges University Purpose 1. Searching the stem cell markers in the cervical tissue. 2. Identifying cervical cancer stem cells with stem cell markers or embryonic stem cell markers. Cervical glandular epithelium (frozen section) CD133 transmembrance protein The function remains unclear CD133 provides an important marker to identify various stem cell populations, especially in brain tumor. Influence on cell polarity, migration and the interaction of stem cells with neighboring cells and/or the extracellular matrix Other candidators (in progress) Bmi-1 Kip p57 Dimethyl-Histon H3(Lys27) GKLF—kruppel-like factors in gut Results MCSP may be positive for reserve cells, however, it is necessary to be tested with more specimens. CD133 is not positive in cervical cancer cells. Oct4 and Sox2 were expressed in the sphere obtained from cervical tissue. Stem cells may be exist in these spheres. Conclusions It might be possible that identifying cervical cancer stem cells with stem cell markers or Embryonic stem cell markers If we could identify the cancer stem cells in the cervix, we would have new better methods for treatment of these diseases. paraffin section Immunohistochemistry Immunocytochemistry frozen section RT-PCR Western-Blot Isolation of cells from tissue and cultivation Preparing samples for electron microscope Acknowledgments * Stem cells Anticancer drug resistance Target of therapy ①Self renewal ②differentiation differentiation Progenitor cells Cancer Stem cells ① Self renewal ② differentiation differentiation Progenitor cells Cancer Stem cells Normal tissue Cancer Malignant transformation +stemness Cancer cells Stem cell system proliferation proliferation Searching stem cell markers From staining pattern of factors Immunohistochemistry Flow cytometry Isolation of stem cells 1. Suspension culture for sphere formation 2