显微镜,双光子FunctImaging_microscopies_2013.pptVIP

Photoactivation Localization Microscopy (PALM) Express protein of interest tagged with a photoactivatable fluorescent protein (eg.g. EOS) in cell Stochastically photoactivate a low density of molecules per frame and localize using Gaussian function Imaging actin tagged with EosFP (photoactivatable protein) Eos-actin TIRF Eos-actin PALM Fibroblasts expressing DsRed “Greening” of DsRed “greening” results from enhancement of green fluorescence and reduction of red fluorescence Clustered T cells after activation Evanescent field excites Ca2+-dependent fluorescence only in a thin layer next