454测序技术FAQ.ppt

* One Fragment = One Bead Emulsification of beads and fragments in water-in-oil microreactors emPCR (emulsion PCR) Amplification Clonal amplification of fragments bound to beads in microreactors One Bead = One Read Sequencing by synthesis TET – turmor suppressor; KRAS cell division regulation; RUNX! Binds to enhancer/promoters; CBL, cell signaling pathways, mutation leads to acute myeloid leukemia; absorption, distribution, metabolism and excretion’ CFTR: Cystic Fibrosis transmembrane conductance regulator ABL1，AKT2，ALK，APC，ATM，BAX， BCL2，BCL6，BLM，BMPR1A，BRAF，BRCA1， BRCA2，CDKN2A，CTNNB1，EGF