心血管科医学范文 毕业设计.docVIP

Y-27632对家兔心肌缺血/再灌注损伤的有益作用 推荐信息：医学论文发表——创新医学网/ 　　作者：梁洪英，郑惠珍，吴纯英，陈智文，吴希春 作者单位：524023 广东湛江，广东医学院生理科学实验室(△生理研究室，通讯作者，▲第一临床学院) 　　【摘要】 目的 研究Rho激酶抑制剂Y-27632对家兔心肌缺血/再灌注损伤(IRI)是否有心脏保护作用。方法 健康家兔18只，体重2.0～2.5 kg，随机分为3组：IRI组、Y-27632(Y)组和假手术(C)组。用结扎左冠状动脉前降支60 min后剪断结扎线恢复血流30 min的方法建立在体心肌IRI模型，Y组于再灌注前5 min，经耳缘静脉注射Y-27632 0.35 mg/kg。检测左心室收缩功能，血浆肌酸激酶(CK)、乳酸脱氢酶(LDH)及心肌缺血、梗死范围。结果 IRI组缺血/再灌注导致左心室舒缩功能受损，左心室收缩峰压(LVSP)、左心室内压最大上升速率(+dp/dtmax)下降，左心室舒张末期压力(LVEDP)升高，CK、LDH的释放增加，心肌梗死范围为(5.3±0.7)%; Y组在再灌注早期LVSP、±dp/dtmax下降的幅度和LVEDP升高的幅度、CK和LDH的释放，均明显低于IRI组，组间比较P均0.05。心肌缺血、梗死范围也明显较IRI组小(缺血：40.5% υs 51.3%，P0.05;梗死：1.7% υs 5.3%，P0.01)。结论 Y-27632对家兔心肌IRI有部分心脏保护作用。 　　【关键词】 缺血/再灌注损伤;Rho激酶;心脏;家兔 　　Effects of Y-27632 on myocardial ischemia-reperfusion injury in rabbits 　　LIANG Hong-ying，ZHENG Hui-zhen，WU Chun-ying，et al.Department of Physiology，Guangdong Medical College，Zhanjiang 524023，China 　　[Abstract] Objective To observe the effects of a Rho kinase inhibitor，Y-27632，on ischemia-reperfusion injury (IRI) in rabbit heart in vivo.Methods Eighteen rabbits were randomly divided into three groups: ischemia reperfusion (IRI) group，ischemia reperfusion plus Y-27632 (Y) group and sham operation (C) group.The animals were subjected to 60 min of left anterior descending coronary artery occlusion and 30 min reperfusion in IRI group.They were treated with Y-27632 at 350 μg/kg by intravenous injection 5 min before reperfusion in Y group.Results Ischemia-reperfusion led to myocardial infarction，the leakage of creatine kinase (CK) and lactate dehydrogenase (LDH) and cardio function decrease.Y-27632 reduced ischemia reperfusion-induced myocardial infarction (1.7% υs 5.3%，P0.01，compared with IR group).It also reduced ischemia reperfusion-induced the leakage of CK and LDH (28.9% υs 34.9% and 45.7% υs 79%，respectively P0.05).Moreover，Y-27632 enhanced post-reperfusion myocardial systaltic function，left ventricular systolic pressure(LVSP)，the maximal rate of left ventricular pressure rise(+dp/dtmax) and left