流式细胞分选原理预案.ppt

流式细胞仪工作原理 ;流式细胞术的原理; ; 基本原理;;; 流 路;FLOW CELL;;; 光 路;; 散射光信号—前向散射光(FS);;; 散射光信号 – 侧向散射光;;;90 Degree Scatter; 光路-荧光信号;荧光染料的特性;Fluorescence emitted by each fluorochrome is usually detected in a unique fluorescence channel. The specificity of detection is controlled by the wavelength selectivity of optical filters and mirrors.;;5 Color Single Laser (488nm)FITC/PE/ECD/PC5/PC7;4 Color Single Laser (488nm)FITC/PE/ECD/PC5;常用荧光染料的特性; 光路 - 滤片特性;;;;Unique 4-Color Single Laser Design;PC7;With Thanks to Dr. Frank Schnieders Gentherapie Gruppe – Abteilung Molekulare Zellbiologie Institut für Medizinische Biochemie und Molekularbiologie Universit?tsklinikum Hamburg-Eppendorf;;减法颜色补偿;全矩阵颜色补偿;;电路及电子系统-光电倍增管;;;单参数直方图;;;软件;Quickset——直观的电压调节;QuickComp——直观的补偿调节;PRISM浓缩柱形图;;;强大的兼容性，易于与医院LIS系统相连;