MicehandledwiththesameprotocolasdepictedinFig.5B.pptVIP

Mice handled with the same protocol as depicted in Fig. 5B were evaluated 2 weeks 2w after treatment. Joseane Morari et al. Diabetes 2014;63:3770-3784 ?2014 by American Diabetes Association Mice handled with the same protocol as depicted in Fig. 5B were evaluated 2 weeks 2w after treatment. A: Hypothalami were obtained for RNA extraction. The cDNA that was produced from total RNA was used in real-time PCR assays to determine the expression of fractalkine CX3CL1 A . Body mass was determined weekly B ; body mass gain was determined at the end of the experimental period C , as was body