乳腺癌干细胞突变与腋窝淋巴结转移的相关性探索.ppt

Correlation of Breast Cancer Axillary Lymph Node MetastasesWith Stem Cell Mutations 乳腺癌干细胞突变与腋窝淋巴结转移的相关性 Background In stem cell theory, breast cancer stem and progenitor cells (BCSCs) are central to cancer proliferation, metastaticpotential,andoutcomemeasures. Breast cancer stemandprogenitor cells have the capacity to initiate tumors, produce multipletumorcell lineages,andmaintain a continuous population of malignant stem cells through self-renewal. Previous research found no oncologic abnormalities in stem and progenitor cells in benign breast tissue; however, oncogenes in thePI3K/Akt signalingpathwaywere identified instemand progenitor cell populations in breast cancer. 乳腺癌干细胞和祖细胞 (BCSCs) 是癌症增殖、转移潜能及转归结局的中心因素。乳腺癌干细胞和祖细胞可分化产生多种肿瘤细胞系，并通过自我更新而维持恶性干细胞群的延续。 在乳腺癌干细胞和祖细胞群中发现 PI3K/Akt 通路涉及到细胞增殖、信号传导、转移潜能等功能，也是目前乳腺癌临床试验青睐的化疗靶点。 Purpose Mutations in oncogenes AKT1, HRAS, and PIK3CA in breast cancers result in abnormal PI3K/Akt signaling and tumor proliferation. They occur in ductal carcinoma in situ,in breast cancers, and in breast cancer stem and progenitor cells (BCSCs). To determine if variability in clinical presentation at diagnosis correlates withPI3K/Akt mutations in BCSCs and provides an early prognostic indicator of increasedprogression and metastatic potential. 在乳腺癌中，癌基因 AKT1、HRAS、PIK3CA 的突变会导致PI3K/Akt 信号通路传导异常和肿瘤异常增殖。这种现象常见于浸润性导管癌以及乳腺癌干细胞及祖细胞 （BCSCs）中。 本实验主要研究乳腺癌干细胞（BCSCs）中 PI3K/Akt 信号通路的突变与乳腺癌的不同临床表现之间的相关性，为判断疾病加速进展和潜在转移提供早期的预后指标。 Methods Womenwithinvasiveduc-tal carcinoma of the breast exceeding 1.0 cm were identified at tumor board meetings and enrolled in the study. Solid-tissuebreastspecimenswerecollectedatthetimeofmastec-tomy or lumpectomy before any adjuvant treatment. Specimens were obtained directly from the operating room andevaluated by a pathologist, and approximately1gof tumor tissuewas immediately transferred to the laboratory for processinginmammaryepithelial cell–specificmedium 研究对象为30例浸润性导管癌的患者（IA 至 IIIB 期），肿瘤直径1cm，术前未行任何辅助治疗。 用流式细胞仪