中华苦荬菜提取物Chinensiolide A的体外抗肿瘤活性.docVIP

中华苦荬菜提取物Chinensiolide A的体外抗肿瘤活性 　　【摘要】 目的：探究中华苦荬菜提取物Chinensiolide A（C15H18O4）对肺腺A549细胞、肝癌Ble-7402细胞及结直肠腺癌LoVo细胞的抗肿瘤活性。方法：用不同浓度的Chinensiolide A处理体外培养的肺腺癌A549细胞、肝癌Ble-7402细胞及结直肠腺癌LoVo细胞24 h后，采用四甲基偶氮唑盐比色法（MTT法）检测Chinensiolide A对该3种细胞生长的影响，计算细胞生长抑制率得出半数抑制浓度（IC50），并观察形态学变化。结果：在1～50 μmol/L浓度范围内，Chinensiolide A对肺腺癌A549细胞、肝癌Ble-7402细胞及结直肠腺癌LoVo细胞都有较强的抑制率，且随着浓度的增大，抑制作用越强，IC50分别是17.79 ?mol/L、25.75 ?mol/L、13.6 ?mol/L。形态学结果显示，Chinensiolide A对上述3种肿瘤细胞均有不同程度的杀伤作用，其作用随浓度的增加而增强。结论：中华苦荬菜提取物Chinensiolide A在体外可有效的抑制肺腺癌A549细胞、肝癌Ble-7402细胞及LoVo细胞的生长，具有较强的抗肿瘤活性。 　　【关键词】 Chinensiolide A； MTT法； 抗肿瘤药； 细胞系； 肿瘤； 体外研究 　　In Vitro Anti-tumor Activity of Extract Chinensiolide A from the Ixeris Chinensis/ZHOU Li，ZHAO Ying，WANG Yu，et al.//Medical Innovation of China，2015，12（34）：109-111 　　【Abstract】 Objective：To explore the antitumor activity of extract Chinensiolide A from the Ixeris chinensis on A549 cells，Ble-7402 cells and LoVo cells.Method：Chinensiolide A was treated with different concentrations of A549 lung adenocarcinoma cells，Ble-7402 liver cancer cells and LoVo cells of colorectal adenocarcinoma after 24 h，the MTT method was used to detect Chinensiolide A influence the three kinds of cell on growth， half inhibitory concentration （IC50） was calculated by cell growth inhibition rate， and the morphological changes were observed.Result：In 1 to 50 ?mol/L concentration range， Chinensiolide A on lung adenocarcinoma A549 cell， Ble-7402 cells of liver cancer and colorectal adenocarcinoma LoVo cells had strong inhibition rate， and with the increase of concentration， the stronger the inhibition，the IC50 was respectively 17.79 ?mol/L，25.75 ?mol/L and 13.6 ?mol/L.Conclusion：Chinensiolide A can effectively inhibit the growth on A549 cells， Ble-7402 cells and LoVo cells in vitro ，it has stronger antitumor activity. 　　【Key words】 Chinensiolide A； MTT assay； Antineplastic drugs； Cell line； Tumor； In vitro study 　　First-author’s address：School of Basic Medicine，Qiqihar Medical University，Qiqihar 1