EGFR和HER3稿7.3.docVIP

胰腺癌EGFR和HER3蛋白表达基因状态分析 （中国医学科学院 北京协和医学院 北京协和医院 病理科，北京 100730）摘要目的 探索胰腺癌EGFR和HER3的基因状态蛋白表达与胰腺癌临床病理因素之间的关系，分析之间的相关性，探讨EGFR与HER3在胰腺癌中的作用。方法 分别采用荧光原位杂交（FISH）和免疫组织化学（IHC）方法，检测55例中国人胰腺癌及癌旁组织EGFR和HER3基因状态和蛋白表达，结合临床病理资料进行统计学分析。结果 在55例胰腺癌中，EGFR FISH阳性有33例（60.0%）。EGFR IHC阳性有34例（61.%）。HER3 FISH 阳性有26例（47.%）。HER3 IHC阳性有4例（7.%）。统计结果并未发现EGFR、HER3蛋白表达与基因扩增之间的存在相关性（P0.05）。EGFR与HER3相互之间在蛋白的表达以及基因状态上也无相关性（P0.05）。EGFR、HER3蛋白表达及基因扩增与患者的性别、年龄、肿瘤大小、肿瘤分化程度、胰周浸润，肝转移均无关（P0.05）。结论 在胰腺癌中，EGFR的蛋白表达显著高于癌旁组织；HER3蛋白表达与癌旁组织无明显差异。胰腺癌中存在较高比例的EGFR、HER3基因扩增。EGFR、HER3蛋白表达与基因拷贝数增加无关。EGFR与HER3相互之间在蛋白表达及基因状态上也不相关。EGFR与HER3在胰腺癌中的作用尚不明确。关键词胰腺癌表皮生长因子受体（EGFR）3；荧光原位杂交免疫组织化学The Gene Status and Protein Expression of EGFR and HER3 in Pancreatic Cancer WANG Ke, YIN Yu-feng, LIANG Zhi-yong, Zeng Xuan (Dept. of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing 100730, China) 【ABSTRACT】Objectives To investigate the gene copy number and protein expression of EGFR and HER3 in pancreatic cancer, try to explore the correlation between these markers, and to determine the relationship between these markers and the pathological features of pancreatic cancer. Methods The 55 cases of paraffin-embedded pancreatic cancer tissues and corresponding tumor adjacent pancreatic tissues from Chinese patients were analyzed by ?uorescence in situ hybridization(FISH) and immunohistochemistry (IHC) for gene copy number and prorein expression, respectively. Results Among all 55 samples, pancreatic cancer EGFR FISH positive, in the form of high polysomy and gene amplification, were present in 33/55 (60.0%) of patients, whereas EGFR protein were over expressed in 34/55 (61.8%) detected by IHC. HER3 increased copy number were present in 26/55(47.3%), the elevated protein level were diplayed in 4/55(7.3%).There was no correlation between gene copy or protein expression of EGFR and HER3 (P0.05).No significant association between EGFR copy number and its corresponding protein level was found(P0.05)