关于波纹龙虾的SCI赏析.pptVIP

Effect of environmental parameters on immune response of theIndian spiny lobster, Panulirus homarus (Linnaeus, 1758) Though influence of salinity, pH, ammonia-N and DO on the immune system of fishes, molluscs, and shrimps have been reported, the influence of such factors on the immune response of spiny lobsters are poorly understood. 3. Results and discussion * Fish Shellfish Immunology (2007) Bindhu Verghese,E.V. Radhakrishnan , Abinash Padhi Navigation Introduction Materials and methods Conclusion and evocation Results and discussion Ｒeferences P.homarus which inhabit the inshore sea along the southern coast of India are exported to Southeast Asian countries. Impulse The high export value of this species increasingly attracts the aquaculturists for farming and fattening. The high stocking density coupled with poor water quality management can lead to devastating consequences on the successful maintenance of these lobsters. + Knowledge of the immune response of lobsters with regard to water quality and stress could provide insight into developing proper management strategies. Here, we report the influence of salinity, pH, DO and ammonia-N on THC, POactivity NBT- reduction(O2-) in the spiny lobster P. homarus. D O —— dissolved oxygen THC —— total circulating haemocytes P O —— phenoloxidase NBT —— nitro blue tetrazolium 2. Materials and methods 2 Test animals Parameters designing Indicators assay Statistical analysis 1 3 4 2.1 Ｔest animals ★ Collect the lobsters from Vizhinjam ★ Acclimatised the lobsters with Perna viridis meat for two weeks ★ Test demands: ① In the intermoult stage ②weight 104+43.88g (25+0.3℃, salinity 35 ‰, pH 7.78+0.2, DO 4.97+1.43 ml /L) All the stress tests were carried out in triplicate . controlled aeration 0.5, 1.5,3.0 0+0.05 ammonia-N concentration ( mg /L) NH4Cl and distilled water 1 5 DO ( mg /L) 1 N HCl or 1 N NaOH 5.0, 9.5 8.0 pH freshwater or saline water 20, 25, 40,45 35 Salinity（‰） environment control Rg