同济-有机物方法精讲.ppt

溶液中有机物分析表征 主要内容 1.常用有机物表征方法 2.有机物分离方法 3.WR最新难降解有机物研究进展 4.沼液相关研究进展 常用有机物表征方法 MW distribution analysis MW distribution analysis has been extensively applied to wastewater samples, and is suitable to be used as a first step in analyzing complex samples. Size distribution analysis has been widely used, normally carried out using ultrafiltration UF or size exclusion chromatography SEC . UF molecular weight cut-offs: 1 kDa, 3 kDa, 5 kDa,10 kDa, 100 kDa, 300 kDa SEC is useful for MW screening of samples before using UF to obtain the separated sample for analysis. There are also some drawbacks: the chemical interaction between the column materials, the solvent, and the organic compounds in SEC can overestimate/underestimate the MW profile. Another disadvantage is that the peaks from SEC can be very difficult to differentiate between in some samples. GPC is when the mobile phase is an organic solvent, and GFC when the mobile phase is aqueous. UF is more suitable for identification of compounds, since large amounts of the sample can be recovered for further characterization. In UF, there are also many factors such as membrane pore size distribution, sample temperature, stirred cell pressure, pH, and membrane materials that can influence the transport of organics through the membranes. 按照Alex et al.提出的有机碳物理尺寸概念 ，有机碳可分为颗粒有机碳 Particulate organic carbon, POC 、胶体有机碳 Colloidal organic carbon, COC 、微胶体有机碳 Fine colloidal organic carbon, FCOC 和溶解性有机碳 Dissolved organic carbon, DOC 。 POC能通过 1.2 μm滤膜但0.45 μm滤膜截留； COC能通过 0.45 μm滤膜但被 0.1 μm滤膜截留； FCOC能通过 0.1 μm滤膜但被 0.025 μm滤膜截留； DOC能通过 0.025 μm滤膜。 Evaluation of on-site biological treatment for landfill leachates and its impact: A size distribution study（WR） Evaluation of on-site biological treatment for landfill leachates and its impact: A size distribution study（WR） HPLC+SEC column: This separation method was effective in revealing the similarities and differences between microbial products from two d