DGGE的主成分析(应用spss软件).docVIP

DGGE图谱的主成分分析 在Excel表中，，列标为泳道，行标为条带，每个泳道中有条带的表示为1，无条带表示为0。 将每一列中全部为0或全部为1的列删掉，只保留部分为0和部分为1的列 打开SPSS，将1，0数据粘贴到SPSS数据表中，最左面一列表示各个处理 选中数据，点击工具栏中analyse——data reduction——factor (5)descriptives中选择：univariate descriptives Initial solution Coefficients (6) extraction中选择： Principal components Correlation matrix/unrotated factor solution Number of factor：2 （7）Scores…..中选择： Save as Variables Regression (8)点击OK 结果中出现factor1-1,factro2-1，同时在output中记录主成分贡献率 (9)点击工具栏中graphs——Scatter 选择simple,点击define Factor1-1进入Y Axix Factor2-1进入X Axix Var00001进入set markers by 点击OK,即出现主成分分析图谱 position for future reference. Installation using special lifting pole rotor 6.4.4.6 rotor lift, box spirit level to check the rotor axis neck of Yang, on both sides of the rotor should be lateral raises and numerical deviation of ≤ 0.10MM, qualified before the official lifting. Hoisting of the rotor dynamic and static clearance should be measured, rotors check clearance to prevent rotor colliding. 6.4.4.7 stationary blade carrier, diaphragm and sealing upper cylinder on the inside and install the mounting bolts, bolts and bolt holes in the surface should be coated with molybdenum disulfide. Follow the manufacturers stamp marked to install good sealing ring and spring. Check the sealing ring and spring plate, shall conform to the technical requirements manufacturers drawings. Bolt: ... Measurement with measurement tools, special tools, temporary connection bolts and connection tool equipped and in good condition. The rotor shaft to have fixed, such as thrust, bearing plates fixed installation, in case of wear of the rotor is too large. The coupling gasket check measurements: measured data processing according to gasket, clean the coupling gasket surface clean and Burr-free, check the coupling gasket thickness data, shim thickness deviation of ≤ 0.02mm, parallel degree deviation of ≤ 0.02mm. Coupling temporary bol