复苏促进因子结合蛋白b的原核表达及对藤黄微球菌促生.docVIP

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复苏促进因子结合蛋白b的原核表达及对藤黄微球菌促生.doc

复苏促进因子结合蛋白b的原核表达及对藤黄微球菌促生

结核分枝杆菌RipB基因的克隆,表达及生物学活性效果Mycobacterium tuberculosis the RipB Cloning,expression and biological activity performance Wang gang, FAN Li-qiang,CHENG An-yang, LI Xiao-ling ,PAN Zhong-xiao, ZHU Qing-qing, MENG Jun State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China Abstract: According to the sequence of RipB, we designed primers, and amplified the gene of RipB with the genome of Mycobacterium tuberculosis as templates. We constructed plasmid pET-21a-RipB, and transformed them into the recombinant E. coli, respectively. According t

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