沉默ABCE1基因电转染卵巢癌肿瘤干细胞对β-榄香烯乳的敏感性.docVIP

沉默ABCE1基因电转染卵巢癌肿瘤干细胞对β-榄香烯乳的敏感性 王 俊，王建中(武汉钢铁(集团)公司第二职工医院肿瘤科，湖北省武汉市 430085) 引用本文：王俊，王建中. 沉默ABCE1基因电转染卵巢癌肿瘤干细胞对β-榄香烯乳的敏感性[J].中国组织工程研究，2016，20(28):4149-4154. DOI: 10.3969/j.issn.2095-4344.2016.28.007 ORCID: 0000-0002-9995-8862(王俊) 文章快速阅读： 文题释义： ABCE1基因β-榄香烯乳的敏感性。 方法：设计合成ABCE1的siRNA序列，通过电转染入卵巢癌肿瘤干细胞。RT-PCR和Western blot检测ABCE1基因沉默后卵巢癌肿瘤干细胞ABCE1 mRNA及蛋白的表达，流式细胞仪检测细胞周期变化，MTT法和细胞克隆形成实验分析ABCE1基因沉默后卵巢癌肿瘤干细胞对β榄香烯乳的敏感性。 结果与结论：①ABCE1基因沉默后卵巢癌肿瘤干细胞的ABCE1基因和蛋白表达明显降低；②ABCE1基因沉默后细胞周期被阻滞在G0/G1期，S期细胞数减少；③ABCE1基因沉默后β榄香烯乳对卵巢癌肿瘤干细胞的增殖抑制率和克隆形成抑制率显著增加；④结果表明，沉默ABCE1基因能显著抑制卵巢癌肿瘤干细胞的增殖能力，并能增强肿瘤干细胞对β榄香烯乳的敏感性。 β-榄香烯乳 主题词： 卵巢肿瘤；肿瘤干细胞；基因沉默；组织工程 ABCE1 silencing enhances sensitivity to beta-eiemene of electrotransferred ovarian cancer stem cells Wang Jun, Wang Jian-zhong (Department of Oncology, the Second Workers’ Hospital of Wuhan Iron and Steel (Group) Company, Wuhan 430085, Hubei Province, China) Abstract BACKGROUND: It is likely to find that ABCE1 plays an important part in the occurrence and progression of cancers through in-depth study. OBJECTIVE: To investigate the effects of ABCE1 silencing on the proliferation and sensitivity to β-eiemene of ovarian cancer stem cells via electrotransfection. METHODS: siRNA sequence of ABCE1 was designed and synthesized, and then was electrotransfection into ovarian cancer stem cells. Subsequently, expressions of ABCE1 mRNA and protein in ovarian cancer stem cells after ABCE1 silencing were detected by RT-PCR and western blot assay; the cell cycle was detected using flow cytometry; the sensitivity of ovarian cancer stem cells to β-eiemene after ABCE1 silencing was analyzed by MTT assay and colony-formation assay. RESULTS AND CONCLUSION: After ABCE1 silencing, expressions of ABCE1 mRNA and protein in ovarian cancer stem cells were significantly reduced. And the cell cycle was arrested in G0/G1 phase, and the number of cells in S phase significantly decreased. Furthermore, the sensitivity of ovarian cancer stem cells to β-