离子交换分离原理及设备总结.ppt

2.0 1.0 0 0 1 2 3 4 5 6 (column volumes) A 280 nm 2.0 1.0 0 0 1 2 3 4 5 6 (column volumes) A 280 nm SOURCE 30RPC FineLINE 200L 400-fold scale up 10 mm diameter column Column: SOURCE 30RPC, a) 10x300 mm (23.6 ml)b) FineLINE 200L, 100x300 mm (9.4 l) Sample: Mixture of Angiotensin II, ribonuclease and insulin Sample load: 0.064 mg/ml medium Eluent A: 0.1% TFA/0.05 M NaCl Eluent B: 0.1% TFA/60% n-propanol Flow rate: 150 cm/h a) 2 ml/minb) 785 ml/min Gradient: 20-70% B, 5 column volumes 切实可行的可放大性 ?KTA? explorer10 BioProcess 23.6-ml column 9.4-litre column 切实可行的可放大性 大型层析柱 多种大小,