Molecular Cloning I Restriction Enzymes, PCR, Plasmid.ppt

304512 Molecular Biology and Recombinant DNA Technology Montarop Yamabhai Schedule: Tue,Th, Fri 10 am- 1 pm, 9-12 July Tue 22 B1205 (11-13) Thr 24 B1114 (11-13) Tue 29 B1205 (11-13) Thr 31 B1205 (11-13) August Fri 1 B1122 (9-12), B1123 (10-12) Thr 14 B1114 (11-13) Fri 15 B1122 (9-12), B1123 (10-12) [Special lecture] Topic Recombinant DNA Technology (July 22,24) Library genomic library cDNA library Phage display library Mutagenesis Basic Bioinformatics Gene Expressions (July 29,31) Production of Recombinant Proteins Reporter-fusion Proteins Immunolocalization Transgenic Animal Modern Methods in Molecular Biology (August 1,14,15) Directed Evolution RNAi Technology Advanced PCR Micro array High Through Put (HTP) Analysis Metagenomics (Special lecture) Reading Molecular Cloning A laboratory manual Sambrook Russell Cold Spring Harbor Laboratory Press c2001 Modern Genetic Analysis Griffiths, Anthony J.F.; Gelbart, William M.; Miller, Jeffrey H.; Lewontin, Richard C. New York: W. H. Freeman Co. ; c1999 Molecular Biology of the Cell Alberts, Bruce; Joh nson, Alexander; Lewis, Julian; Raff, Martin; Roberts, Keith; Walter, Peter New York and London: Garland Science ; c2002 Molecular Cell Biology Lodish, Harvey; Berk, Arnold; Zipursky, S. Lawrence; Matsudaira, Paul; Baltimore, David; Darnell, James E. New York: W. H. Freeman Co. ; c1999 Cell and Molecular Biology: Concepts and Experiments, 5th Edition Gerald Karp, Formerly of the Univ. of Florida, GainesvilleISBN 978-0-470-04217-5, ?2008, 864 pages Evaluation Assignments 40% Select a company that sale any “advanced” biotech products that you like. Then, write a “product review” describing this product in as much detail as possible but no more than one A4 page Exam 60% Opeb book, 3 hrs. ? 22 August 2008? Advanced Recombinant DNA Technology Library genomic library cDNA library Phage display library Mutagenesis Site-directed mutagenesis Random mutagenesis Bioinformatics PCR Library A colle