igem6.2.pptVIP

Shape Shifting L-forms L-forms are cell wall-deficient bacteria which are able to grow as spheroplasts or protoplasts. Part 1 Naked bacteria L-form division vesiculation growth tubulation blebbing Part 1 Naked bacteria Growth Video 1. Showing L-forms with HBsu-GFP tag growing in size from around Part 1 Naked bacteria Part 1 Naked bacteria blebbing（出泡） division of L-form cells via blebbing Showing larger size L-forms 3.5μm - 6μm dividing through forming transient bulges around the cell membrane, followed by a series of small bodies erupting from the membrane forming apparent progeny. Tubulation The cell forms a protrusion A blunt protrusion Part 1 Naked bacteria Vesiculation This figure shows an often-used method of division by L-forms 2μm. They undergo a similar process as larger L-forms. They usually gave rise to progeny of equal sizes. Part 1 Naked bacteria Characterisation of Growth and Division Part 1 Naked bacteria Kill Switch To further highlight the osmotic instability of L-forms Part 1 Naked bacteria Part 2 L-form switch Rod to L-form Switch BioBrick Part 2 L-form switch GENE CIRCUIT L-form switch BioBrick B. subtilis LR2 chromosome Part 3 Genome shuffling fluorescently tagging our L-form genomes with two different colours fusing the cells and observing whether the two colours mixed. We created and used the parts BBa_K1185001 and BBa_K1185002, which marked bacterial genomes with HBsu-sfGFP a green colour and HBsu-RFP a red colour respectively. Part 3 Genome shuffling Modelling Part 3 Genome shuffling Results Part 4 L-forms in plants Figure. Chinese cabbage seedlings inoculated with L-forms growing on a mutishage and Skoog plate. maintain and increase the productivity of agricultural land wherever possible directly modify plants inoculate with regular bacteria inoculate plants with L-forms Part 4 L-forms in plants Part 4 L-forms in plants prove that L-forms can grow in plants（detect L-forms in plants） β-glucuronidase assay Enzyme-linke