T淋巴瘤侵襲转移诱导因子1与胃癌细胞侵袭移行能力的相关性研究.doc

T淋巴瘤侵袭转移诱导因子1与胃癌细胞侵袭移行能力的相关性研究 　　作者：朱金明　余佩武　李翠芳　赵永亮 作者单位：1第三军医大学附属西南医院　普通外科 (重庆　400038) 2邢台市第五人民医院　内二科　(河北　邢台　054027) 　　【摘要】 目的：检测T淋巴瘤侵袭转移诱导因子1(Tiam 1)在胃癌细胞株中的表达并分析其与胃癌细胞侵袭、移行能力的关系。方法：采用层黏连蛋白黏附法，从胃癌MKN-45细胞株(MO)中筛选获得高黏附亚株(MH)和低黏附亚株(ML)。应用RT-PCR和SABC免疫组化技术分别检测Tiam 1 mRNA与蛋白在MO、ML、MH细胞中的表达。应用Boyden小室测定MO、ML、MH细胞的体外侵袭、移行能力并分析其与Tiam 1表达间的关系。结果：胃癌MKN-45细胞高黏附亚株(MH)的体外侵袭、移行能力均较MKN-45细胞(MO)及其低黏附亚株(ML)为强(P 0.05)，但MO与ML细胞间无差异(P0.05)。Tiam 1 mRNA和蛋白在MH细胞中的表达均较其在MO和ML细胞中的表达为强(P0.05)，但在MO与ML细胞中的表达无差异 (P 0.05)。Tiam 1蛋白和mRNA表达水平与胃癌细胞体外侵袭、移行能力呈正相关 (P0.05或P0.01)。结论：Tiam 1基因表达水平升高有可能促进胃癌细胞侵袭、移行能力的增强。 　　【关键词】 胃肿瘤　T淋巴瘤侵袭转移诱导因子　侵袭转移 　　Correlation between Tiam 1 and the invasive and migratory potential of gastric cancer cells 　　ZHU Jin-ming1, YU Pei-wu1, LI Cui-fang2, ZHAO Yong-liang1 　　1Department of General Surgery, Southwest Hospital, Third Military Medical University(Chongqing 400038,China) 　　2Department of Internal Medicine, Fifth Peoples Hospital, Xingtai, HeBei Province(Xingtai 054027, China) 　　【ABSTRACT】 Objective: To investigate the expression of T lymphoma invasion and metastasis inducing factor 1 (Tiam 1) in gastric cancer cell lines and analyse the correlation between Tiam 1 and the invasive and migratory potential of gastric cancer cells. Methods: Two subpopulations of human gastric cancer cell line MKN-45 (MO), less adhesive subgroup (ML) and higher adhesive subgroup(MH) were separated by laminin adhesion method in vitro. RT-PCR and SABC immunohistochemical technique were applied to detect the expression of Tiam 1 mRNA and protein in MO, ML and MH cells. The invasive and migratory potential of MO, ML and MH cells in vitro were observed by Boyden chamber, simultaneously the correlation between the expression of Tiam 1 and the invasive and migratory potential of gastric cancer cells also was analysed. Results: The invasive and migratory potential of MH cells was much higher than that of MO and ML cells (P0.05), so did the expression of Tiam 1 mRN