醒神噴鼻液对脑出血大鼠脑组织的钙离子浓度线粒体膜电位的影响研究.docVIP

———————————————— 作者简介：李翎，广州中医药大学第一附属医院，副研究员，主要从事中药药理研究。 基金项目：广东省科技厅攻关课题，项目编号2007B031408005 醒神喷鼻液对脑出血大鼠脑组织的钙离子浓度、线粒体膜电位的影响研究 李翎1　方永奇　邹衍衍 何玉萍 （广州中医药大学第一附属医院，广东广州 510405） 摘要：目的：观察醒神喷鼻液对脑出血模型大鼠脑组织的钙离子浓度、线粒体膜电位的影响。方法：SPF级SD大鼠随机分为醒神喷鼻液高、中、低剂量组、醒脑静组和模型对照组，各组大鼠滴鼻给药3天后，造脑出血模型，进行神经症状评分判断模型成功与否，造模成功动物再给药1天，24h后断头取脑。应用流式细胞术测定脑组织的钙离子浓度和线粒体膜电位。结果: 醒神喷鼻液能明显降低脑出血大鼠的神经症状评分，并呈一定量效关系。高、中剂量能降低模型大鼠脑组织的钙离子平均荧光强度，高剂量能升高线粒体膜电位的平均荧光强度。结论：醒神喷鼻液可抑制脑出血时脑组织的钙离子超载、保持线粒体膜电位的稳定，减轻脑损害程度，其作用机制可能与钙拮抗作用和细胞凋亡机制有关。提示脑出血时药物对脑细胞有保护作用。 关键词：醒神喷鼻液；脑出血；钙离子浓度；线粒体膜电位 The effect of Xingshen nasal spray on calcium concentration and mitochondrial membrane potential in rat brain after intracerebral hemorrhage Li Ling1, FANG Yong-qi, ZOU Yan-Yan, HE Yu-ping (1 The First Affiliated Hospital Guangzhou University of T C M，Guangzhou 510405，China) Abstract:Objective:To investigate Xingshen nasal spray on calcium concentration and mitochondrial membrane potential in rat brain after intracerebral hemorrhage.Methods:SD rats in SPF were randomly divided into the high,medium and low-dose group of Xingshen spray nasal、Xingnaojing group and model control group. Administering the rats intranasally 3 days,ICH model were maked. Applying the neurological symptom score to determine the model,Successful model of rats were administered one day and brains were removed after 24 h.Results:The Xingshen nasal spray significantly reduced neurological symptom score in rats after intracerebral hemorrhage,and showed a definite dose-effect relationship.High、medium dose decreased mean fluorescence intensity of calcium in the brain,while high doses increased the average fluorescence intensity of mitochondrial membrane potential.Conclusion:The Xingshen nasal spray may inhibit calcium overload、maintain the stability of mitochondrial membrane potential and reduce the extent of brain damage during intracerebral hemorrhage.Which mechanism may be related to calcium antagonism and the mechanisms of apoptosis.It can be concluded that drugs had a prote