原创力文档Enzymatic ransformation of Rg3 to Rh2 using newly isolated Fusarium proliferatum ECU2042.doc
- 1、本文档共6页，可阅读全部内容。
- 2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
- 3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
查看更多
Enzymatic ransformation of Rg3 to Rh2 using newly isolated Fusarium proliferatum ECU2042
Abstract
Journal of Molecular Catalysis B: Enzymatic 38 (2006) 113–118
Enzymatic transformation of ginsenoside Rg3to Rh2using
newly isolated Fusarium proliferatum ECU2042
Jin-Huan Sua, Jian-He Xua,?, Wen-Ya Lub, Guo-Qiang Linb,?
a Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering,
East China University of Science and Technology, Shanghai 200237, China
b Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
Received 13 September 2005; received in revised form 1 December 2005; accepted 10 December 2005
A new soil isolate, Fusarium proliferatum ECU2042, was found capable of producing a ginsenoside Rg3hydrolase (a special -glucosidase)
which is useful for production of a very potent antitumor agent Rh2. By investigating its fermentation progress, the optimal time for -glucosidase
biosynthesis was determined as 72 h. The crude -glucosidase was partially puri?ed by 49.4-fold from the cell-free extract with an activity yield
of 13%. The catalytic performance of the partially puri?ed -glucosidase was examined, giving optima of pH and temperature at pH 5.0–6.0
and 50 ?C. It was found that the F. proliferatum -glucosidase can also hydrolyze cellobiose, but not active towards a frequently used arti?cial
substrate p-nitrophenyl -d-glucopyranoside (pNPG). In a lab-scale preparation, the bio-hydrolytic reaction was carried out at 50 ?C for 24 h in
50 ml NaAc/HAc buffer (100 mM, pH 5.0) containing 50 mg Rg3and 12 mg lyophilized crude enzyme extract, giving the ginsenoside Rh2in 60.3%
conversion.
? 2005 Elsevier B.V. All rights reserved.
Keywords: Ginseng saponin; Ginsenoside Rg3; Ginsenoside Rh2 ; -Glucosidase; Fusarium proliferatum
1. Introduction
Saponins, glycosides with steroids or triterpenes as agly-
cons, are an important class of physiologically active compounds
occurring in many herbs. In recent years, the sugar chains of the
saponins were found to
文档评论（0）