PCR enhancrs.docVIP

PCR 增强剂 PCR enhancer?· DMSO and glycerolRecommended by a number of authors to improve amplificationefficiency and specificity of PCR. However, in the multiplex reactionthese enhancers gave conflicting results. For example, 5% DMSOimproved the amplification of some products , decreased theamount of others and some loci were not affected at all. The sameobservations were made with 5% glycerol.· Bovine Serum Albumin (BSA):100 μg/ml - 1 ug/ul, 0.01% - 0.1% (w/v)The addition of albumin to tissue DNA samples increases theamount of DNA generated by neutralizing many deleterious factors