2015中期考核.pptxVIP

扬 州 大 学 毕业设计中期考核汇报 指导老师：鲁玉柱 汇报人：周春艳 扬州大学 生物科学与技术学院 生技1301班 水稻miR396启动子的表达特性 目 录1.background4.问题产生的原因分析2.assignment5.结论及其他3.6.未来实验计划1. backgroundAbout miR396:Based on the computational and experimental means, miR396 was cloned and identified in both Arabidopsis and rice. It is a very highly conserved microRNA in plants. Its target genes, GRF(Growth Regulative Factor), were believed as GA-induced gene. However, the mechanism of this induction in unknown.Our previous work showed that the transgenic rice over-expressing miR396 presented as a dwarf phenotype.The phenotype of rice over-expressing miR396Our some previous results indicated that the reason of the positive response of GRF to GA is caused by negative response of miR396 to GAThe expressional pattern of a given gene is a significant indicator for exploring the function of this gene.In this study, we want to investigate the expressional pattern of miR396 by the method of driving the expression of a reporter gene, GUS, by the promotor of miR396.Part identified sequence of the promoter of miR396It is difficult to directly detect the expression of microRNA by Northern blot, but it is very easy to observe the expressional pattern of a reporter gene. By the acknowledge that a gene’s expressional feature is determined by its promoter, the promoter of miR396 was successfully cloned and constructed into expressional vector to drive the expression of GUS2. The main purpose of my studyTo investigate the expressional pattern of GUS, whose expressional pattern is controlled by the promotor of miR396To investigate whether the expression of GUS is negatively regulated by GATo investigate the expressional of GUS under various stresses, including biotic and abiotic stresses. 3. Some results of my current work主要研究内容：miR396的表达分析? 获得一个过表达miR396的转基因水稻。? 12个水稻GRF （Growth Regulating Factor）中的11个是miR396的靶基因受miR396的负调节。我们发现，先前报道的水稻GRF基因正响应GA的原因是因为miR396负响应GA所引起的。? 利用突变体slr1（水稻DELLA基因功能丧失型）和miR396的过表达突变体，还发