Cytodext-3微载体藻酸钠水凝胶作为可注射性支架构建组织工程软骨的可行性..docVIP

Cytodext-3微载体/藻酸钠水凝胶作为可注射性支架构建组织工程 软骨的可行性 尹合勇1，2，孙 振1，李 盼3，余晓明1，徐亦驰1，孙 逊1，2，肖 波1，王 玉1，汪爱媛1，郭全义1，徐文静1，卢世璧1，彭 江1 (1解放军总医院全军骨科研究所，北京市 100853；2南开大学医学院，天津市 300071；3河北医科大学，河北省石家庄市 050017) 引用本文：尹合勇，孙振，李盼，余晓明，徐亦驰，孙逊，肖波，王玉，汪爱媛，郭全义，徐文静，卢世璧，彭江. Cytodext-3微载体/藻酸钠水凝胶作为可注射性支架构建组织工程软骨的可行性[J].中国组织工程研究，2016，20(34):5104-5109. DOI: 10.3969/j.issn.2095-4344.2016.34.014 ORCID: 0000-0002-0033-9501(尹合勇) 文章快速阅读： 文题释义： 微载体培养：是一种特殊的细胞培养技术，因其具有扩增种子细胞并维持细胞表型的特点，成为动物细胞大规模培养的新方法。目前，微载体数量颇多，包括聚苯乙烯微载体、液膜微载体、中空多孔微载体、大孔明胶微载体及磁性微载体等。Cytodex-3微载体是目前使用比较广泛的一种商业化微载体。 Cytodext-3微载体/藻酸钠水凝胶：njectable tissue-engineered cartilage using cytodex-3 microcarrier and alginate hydrogel Yin He-yong1, 2, Sun Zhen1, Li Pan3, Yu Xiao-ming1, Xu Yi-chi1, Sun Xun1, 2, Xiao Bo1, Wang Yu1, Wang Ai-yuan1, Guo Quan-yi1, Xu Wen-jing1, Lu Shi-bi1, Peng Jiang1 (1PLA Institute of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China; 2School of Medicine, Nankai University, Tianjin 300071, China; 3Hebei Medical University, Shijiazhuang 050017, Hebei Province, China) Abstract BACKGROUND: Alginate hydrogel and microcarrier both can be used as injectable scaffolds, but their shortcomings such as poor mechanical property and poor plasticity remain unresolved. OBJIECTIVE: To explore the feasibility of constructing an injectable tissue-engineered cartilage with cytodex-3 microcarrier/alginate hydrogel composite. METHODS: Injectable cytodex-3 microcarrier/alginate hydrogel composite scaffold and injectable alginate hydrogel scaffold were established, and the mechanical properties of the two scaffolds were detected. Chondrocytes-seeded cytodex-3 microcarrier was obtained after incubated in the bioreactor, and then composited with alginate hydrogel as experimental group; chondrocytes were co-cultured with alginate hydrogel as control group. Subsequently, cell viability and ability of DNA and glycosaminoglycan synthesis were detected. RESULTS AND CONCLUSION: Th