ankom protocol- cardelús lab（ankom协议——cardelus实验室）.docVIP

Ankom Protocol The Ankom Fiber Analyzer utilizes a series of extractions to determine the fiber content of a plant sample. Each of the extractions should be done in the order of NDF (Neutral Detergent Fiber), ADF (Acid Detergent Fiber), and then ADL (Acid Determined Lignin). The NDF solution is just soapy water. During NDF, the fraction that is washed off contains soluble cell contents like carbohydrates, lipids, pectin, starch, soluble proteins and non-protein nitrogen. The fraction that is left in the bag contains hemicellulose, proteins bound to the cell walls, cellulose, lignin, and