reverse transfection methods- sabatinilab（sabatinilab reverse transfection methods -）.docVIP

Reverse Transfection Guide ? Table of Contents Introduction Page 1 Gelatin-DNA Method: Page 2 Gelatin preparation, DNA purification and Microarray printing Page 2 Cell Preparation Page 3 Reverse-transfection of printed microarrays Page 4 Detection Methods Page 5 Immunofluorescence Page 6 Autoradiography Page 6 Western Blots Page 6 Tips and Information Page 7 Alternative method: Lipid-DNA Method Page 9 References Page 11 How to contact us Page 11 This guide describes a microarray-based system for the functional analysis in mammalian cells of many genes in parallel. Mammalian