Anamperometricglucosebiosensor选编.ppt

Contents Abstract Introduction Experimental Results and discussion Conclusions Abstract An amperometric glucose biosensor based on enhanced and fast direct electron transfer (DET) of glucose oxidase(GOx) at enzyme dispersed multiwalled carbon nanotubes/graphene oxide (MWCNT/GO) hybrid biocomposite was developed. The fabricated hybrid biocomposite was characterized by transmission electron microscopy (TEM), Raman and infrared spectroscopy (IR). The TEM image of hybrid biocomposite reveals that a thin layer of GOx was covered on the surface of MWCNT/GO hybrid composite. IR results validate that the hybrid biocomposite was formed through the electrostatic interactions between GOx and MWCNT/GO hybrid composite. Further, MWCNT/GO hybrid composite has also been characterized by TEM and UV–visible spectroscopy. A pair of well-defined redox peak was observed for GOx immobilized at the hybrid biocomposite electrode than that immobilized at the MWCNT modified electrode. The electron transfer rate constant (Ks) of GOx at the hybrid biocomposite was calculated to be 11.22 s?1. The higher Ks value revealed that fast DET of GOx occurred at the electrode surface. Moreover, fabricated biosensor showed a good sensitivity towards glucose oxidation over a linear range 0.05–23.2 mM. The limit of detection (LOD) was estimated to be 28 μM. The good features of the proposed biosensor could be used for the accurate detection of glucose in the biological samples. Introduction Glucose oxidase (GOx), a flavin enzyme with molecular weight of 150–180 kDa, has been extensively used to monitor the blood glucose levels in diabetics for its catalytic ability to glucose. However, the realizing of DET between GOx and the electrode is extremely difficult due to the active site of GOx, flavin adenine dinucleotide (FAD), being deeply embedded within a protective protein shell. Introduction There have been great challenges in the development of highly sensitive and sophisticated glucose biose