thermo原子吸收最新培训资料 Atomic Absorption Theory.ppt

The AAS Technique The Strengths of AAS Theory of Atomic Spectroscopy Principle of Atomic Absorption Schematic of an AAS system Optical system Optical system Why an Echelle Single/Double Beam M Series Optics Hollow Cathode Lamp Lamp Power Supplies Flame-Burners Flame- Burner Jaw Profile Flame-Nebuliser Flame-Spray chamber Flame-Gas control Flame Types Furnace-Heat Furnace-Temperature control Furnace-Heat Phase Ash-atomize temperature optimization Furnace-Cuvette Background Correction QuadLine B/C B/C-ZEEMAN B/C- AC Zeeman B/C- SelfReversal Starting An Analysis 配备一系列水溶液标准，石墨炉法可由自动进样器配置 仪器操作条件的最佳化，波长、灯电流、负高压，火焰法：燃气比、燃烧器高度，石墨炉法：升温程序、扣背景、基体改进剂等 在最佳条件下测水溶液标准 测定吸光度 以浓度与吸光度绘制校准曲线 Calibration Graph SOLAAR MQZe SOLAAR MQZe ID90 Dilutor ID90 Dilutor ID90 Dilutor 0.00 2.00 3.00 4.00 5.00 6.00 0.000 0.101 0.202 0.304 0.408 0.516 0.632 1.0 2.0 3.0 4.0 5.0 6.0 Conc mg/L 0.2 0.4 0.6 Abs. 1.00 Conc Abs The perfect environmental AA System Vapour and Zeeman Furnace Typical elements : Al, Sb, As, Cd, Cr, Co, Cu, Fe, Pb, Mn, Ni, Se, Ag, Tl, Sn (EPA Method 200.9) : Hg (EPA Method 245.1) Vapour Cell Plane mirror Vapour Toroid Mirror D2 Lamp Rear Beam Selector HCL Carousel Zeeman Furnace Plane Mirror Furnace Toroid Mirror ID90在线稀释器 Atomic Absorption Theory Atomic Absorption Training 67 elements 67 elements flame ppm Furnace sub-ppb Multiple Applications Metals in Solution Alan Walsh 1955 Powerful Technique AAS 火焰原子吸收法 仪器结构较简单，操作方便，分析速度快 采用空心阴极灯光源，共存元素及基体光谱干扰少 分析结果准确，稳定性好 分析成本低 石墨炉原子吸收法 非常低的检出极限 Sub-ppb 样品用量少 仪器结构紧凑 共存元素及基体光谱干扰少 Excited states E n e r g y E n e r g y Ground state Atom 1 Atom 2 每个原子有独自的能级排列，通常原子处于基态，当提供能量时，原子会跃迁到激发态 Incident Radiation (Io) Transmitted Radiation (It) Excited State Atom absorbs some of the incident light Beer’s Law : Absorbance, A is proportional to concentration A=log10 Io / It Ground State 当元素灯发射的与被测元素特征波长相同的光辐射穿过一定厚度的原子蒸气时，光的一部分被原子蒸气吸收，检测系统测得特征光谱被基态原子吸收后的辐射能量。 Hollow cathode la