慢性阻塞性肺病TNFA和LTA基因等位基因变异分析.doc

慢性阻塞性肺病TNFA和LTA基因等位基因变异分析 　　作者：张瑛琪 卢宝全 作者单位：唐山市工人医院呼吸内科，河北 唐山 063000 　　【摘要】目的 中国北方汉族人群中研究TNF超家族基因等位基因变异是否与慢性阻塞性肺病(COPD)相关联。方法 以50例COPD患者为研究对象，应用聚合酶链反应限制性片段长度多态性方法研究TNF超家族基因(TNFA和LTA)等位基因变异分布。结果 TNFA基因多态性位点308G/A COPD组和对照组比较AA基因型频率分布差异显著(Χ2=7.111,P0.01),OR值为10.756(95%CI为9.875～12.640)。308G/A多态性位点A等位基因频率差异显著(Χ2=8.219,P0.01);LTA基因+252A/G多态性位点AG基因型频率分布COPD组与对照组比较差异显著(Χ2=11.974，P0.01)，OR值为4.373(95%CI为3.301～6.872)。LTA基因+252 A/G多态性位点G等位基因频率差异不显著。在COPD患者组中TNFA基因GG正常基因型和LTA基因AG杂合基因型结合个体频率比对照组显著增高(Χ2=4.10，P0.05)。结论 中国北方汉族人群LTA基因等位基因变异、TNFA基因多态性变异组合与COPD相关联。 　　【关键词】 慢性阻塞性肺疾病 TNFA基因 LTA基因 　　Analysis of TNFA and LTAgene allele variation in COPD 　　ZHANG YingQi，LU BaoQuan. 　　Department of Respiration of Workers′ Hospital of Tangshan, Tangshan 063000, Hebei, China 　　【Abstract】 Objective To research whether TNFgene allele variation is associated with chronic obstructive pulmonary disease (COPD) of Northern Han Chinese. Methods TNFsuperfamilygene (TNF and LTA) allele variation distribution were determined by PCRrestrictive segement length polymorphism method in 50 COPD cases. Results There was significant difference in AAgenotype frenquency distribution between COPD group with TNFgene polymorphism space point 308G/A and control group 〔(Χ2=7.111, P0.01), OR value for 10.756 (95%CI for 9.875～12.640)]. An allele of polymorphism space point 308G/A frequency also had significant difference (Χ2=8.219, P0.01); LTA gene +252A/G polymorphism space point AG allele genotype frequency had a significant difference between the two groups (Χ2=11.974, P0.01), OR value for 4.737 (95%CI for 3.301～6.872). LTA gene +252A/G polymorphism space point G allele frequency showed no significant difference. In COPD group TNFAgene GG normal genotype and LTAgene AG hybrid gene combinated individuals were significantly higher than that of control group (Χ2=4.10, P0.05). Conclusions LTAgene allele variation and TNFAgene polymorphism variation are associated with COPD.