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The role of cellular oxidative stress in regulating glycolysis energy metabolism in hepatoma cells.docx

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The role of cellular oxidative stress in regulating glycolysis energy metabolism in hepatoma cellsDong-yun Shi1,2, Fei-zhou Xie1, Chao Zhai2, Jeremy S Stern3, Yang Liu4and Shan-lin Liu1,41? Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Free Radical Regulation and Application Research Center of Fudan University, Shanghai 200032, PR China2? Pharmaceutical Science Research Division, School of Health and Life Sciences, Kings College London, London, SE1 9NH, UK3? Department of Neurology, St Georges Hospital, London, SW17 0QT, UK4? Free Radical Life Science Research Center, Zhejiang University, Hangzhou 310027, PR Chinaauthor email corresponding author emailMolecular Cancer 2009, 8:32doi:10.1186/1476-4598-8-32The electronic version of this article is the complete one and can be found online at: /content/8/1/32Received:4?March?2009Accepted:5?June?2009Published:5?June?2009? 2009 Shi et al; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.AbstractBackgroundThe Warburg effect has been found in a wide spectrum of human cancers, however the underlying mechanisms are still unclear. This study aims to explore the role of cellular oxidative stress in relation to glycolysis and the Warburg effect in hepatoma cells.MethodsVarious cell lines combining environmental hypoxia was used as an in vitro model to mimic tumor microenvironment in vivo. Superoxide dismutases (SOD) and xanthine oxidase (XO) gene transfection were used to produce various cellular redox levels. 2,7-dichlorofluorescin (DCF) fluorescence and ESR spectrum were used to detect cellular reactive oxygen species (ROS).ResultsWe found that endogenous or exogenous interference with the cellular oxidative stress can sensitively regulate glycolysis and the