长爪沙鼠生物净化体会-中国比较医学杂志.docVIP

技术方法 长爪沙鼠的生物净化技术 戴方伟1，宋晓明2，卢领群1，周莎桑1，李长龙3, 马伟锋1，柯贤福1，楼 琦1，吕 宇1，萨晓婴1 (1 浙江省医学科学院实验动物中心, 杭州 310013;2 杭州师范大学实验动物中心, 杭州 310036；3 首都医科大学基础医学院实验动物学系，北京 100069) 【摘要】目的 实现长爪沙鼠微生物质量升级和实验动物化目标，需对长爪沙鼠实施生物净化。方法 在长爪沙鼠生物净化过程中，采取孕鼠选择和临产期判断、无菌剖腹产、仔鼠代乳以及独立通风笼盒（IVC）饲养管理等措施，比较了ICR小鼠、SD大鼠、长爪沙鼠代乳和饲料中添加营养成分对仔鼠代乳的影响。结果 本研究共实施长爪沙鼠无菌剖腹产达85胎次，通过ICR代乳鼠代乳并成功断乳167只。结论 本研究顺利实现了长爪沙鼠生物净化，形成的关键技术有望为我国特色实验动物资源的生物净化提供参考。 【关键词】长爪沙鼠；生物净化；微生物 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2014.004.000 Biological cleaning technologies for Mongolian gerbils DAI Fang-wei1, SONG Xiao-ming2, LU Ling-qun1, ZHOU Sha-sang1, LI Chang-long3, MA Wei-feng1, KE Xian-fu1, LOU Qi1, LV Yu1, SA Xiao-yin1 (1. Center of Laboratory Animals, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China;2. Center of Laboratory Animals, Hangzhou Normal University, Hangzhou 310036 3. Department of Laboratory Animal Sciences, Capital University of Medical Sciences. Beijing 100069) [Abstract] Objective To obtain higher microbiological quality of Mongolian gerbil colonies and realization of experimental animalization by biological cleaning. Methods We conducted biological cleaning of Mongolian gerbils by selection of pregnant gerbils, perinatal judgment, aseptic Caesarean section, pup milk replacer and breeding in individually ventilated cages. We also compared the results by milk replacer for pup gerbils from lactating ICR mice, rats as well as gerbils. Results During the present study, 85 Caesarean operations were carried out and a total of 167 gerbils were weaned. Conclusions We have successfully achieved the biological cleaning from a Mongolian gerbil colony in Zhejiang province, and the key techniques may provide useful reference for biological cleaning of special laboratory animal species in China. [Keywords] Mongolian gerbil; Meriones unguiculatus; Biological cleaning; Microbe 长爪沙鼠(Meriones unguiculatus)属于啮齿目、沙