Genomesequencingandassembling.pptVIP

Genome sequencing and assembling Chitta Baral Basic ideas and limitations Current lab techniques can sequence small (say 700 base pairs) DNA pieces. Use restriction enzymes to cut DNA pieces Sort pieces of different sizes using gel electrophoresis and use the sorting to read them Mapping and Walking Sequence one piece, get 700 letters, make a primer that allowed you to read the next 700, and work sequentially down the clone Estimate for human genome sequencing using this method: 100 years Shotgun sequencing (introduced by Sanger et al. 1977) for sequencing genomes Obtain random sequence reads