HPLC Analysis of Ionic Compounds Seton Hall 高效液相色谱法分析西顿霍尔离子化合物.pptVIP

HPLC Analysis of Ionic Compounds Nicholas H. Snow Seton Hall University HPLC of Ionic Compounds Ion Suppression Ion Exchange Chromatography (IEX or IEC) Ion Pair Chromatography (IPC) Weak Acid Equilibrium LeChatelier Principle Ion Suppression Weak acid - Use pH 2-3 acetic or phosphoric acid Weak base - Use pH 8 ammonium hydroxide, sodium carbonate Ionization is suppressed Neutral molecules chromatographed by reversed phase LC Ionization Suppression Effect Strong Acids and Salts100% Ionized IEC Mechanism Anion Exchange Ion Exchange Ion Exchange Ion Exchange Parameters Increasing pH Increases i