Excited-statereaction.ppt

Principles of Fluorescence Spectroscopy Chemistry Department XMU Reference books 陈国珍等人编, 荧光分析法， 第二版，科学出版社，1990 J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum Press, New York, 1999 (Ed.2) 1983 (Ed.1) J. R. Lakowicz ed. Topics in Fluorescence Spectroscopy, 1-5, Plenum Press, New York 陈国珍等人编，荧光分析进展，厦门大学出版社，1992 B. Herman, Fluorescence Microscopy 2 ed. University of North Carolina Research papers Course Requirements: One term paper, reviews including relevant research papers from 1998 to 2003. Self-chosen topic. 20% One oral report (same topic as term paper) （12 mins report, 3 mins defence) 20% One translation (From Science, related to luminescence) 10% Send to xqguo@jingxian.xmu.edu.cn One test (basic principles) 50% 关于教学 教学方式：讲授与研修、论文报告 评价：教师评价与学生互评结合 总课时：54 INTRODUCTION TO FLUORESCENCE Introduction to Fluorescence 1.0 Introduction 1.1 Phenomenon of Fluorescence 1.2 Excitation and deactivation of molecule 1.3 Models of molecular emission 1.4 Characteristics of fluorescence emission 1.5 Characteristics of fluorophore 1.6 Time scale of Fluorescence 1.7 Intensity and Concentration 1.8 Fluorophore Fluorimetry Application 1.1 Phenomenon of Fluorescence The phenomenon of fluorescence described by Herschel 1.2 Excitation and deactivation of molecule 电子跃迁与电子激发态 电子的多重态 1.2.2 Jablonski diagram Keys for understanding the Jablonski diagram 1.3 分子发光类型 1.3.2 荧光的类型及其发射过程 Excimer Characteristics of Excimer Characteristics of Excimer Characteristics of Excimer Time related fluorescence spectra 迟滞荧光 瞬时荧光与迟滞荧光 从分析的觉度，更关注瞬时荧光。 分析常应用的是常规荧光。 二聚体荧光应用为探针，表征微环境粘度等。 Stokes荧光、反Stokes荧光、共振荧光 Multiphoton excitation Emission spectra of DPH with one (287 nm) and three-photon (860 nm) (excitation Scatter 1.4 Characteristics of fluorescence emission 1.4.1 stokes’ shift 1.4.2 Emission spect