Stimulation of In Vivo Angiogenesis Through Dual 在体内血管生成通过双.pptVIP

Stimulation of In Vivo Angiogenesis Through Dual Growth Factor Loaded Hyaluronic Acid Hydrogels Celeste Riley Mentor: Dr. Robert Peattie Department of Chemical Engineering Importance Engineered tissues Organ transplants Ischemic Pathologies (Diabetes) Components of the Project Preparation of Hydrogels Surgery on living mouse models Count formed blood vessels and analyze data Hydrogel Preparation Natural or Synthetic Biocompatible Crosslinked to form a gel-like substance Dehydration Hydrogel Preparation Glycosaminoglycan Complexes H20 molecules Chief component of extracellular matrix Contributes to cell proliferation and migration Addition of Growth Factors Signaling proteins Bind receptors of target cells Promote differentiation and maturation Addition of Growth Factors Vascular Endothelial Growth Factor (VEGF) Angiopoietin 1 (Ang-1) Goal of Project Make hydrogel from hyaluronic acid Impregnate hydrogel with growth factors Ang-1 and VEGF Dehydrate and implant hydrogel into mouse ear Determine if implant, loaded with growth factors, stimulates angiogenesis Project Setup Spontaneous Growth Original Vessels Individual combinations: control cases (chart) Treatment Cases Determining Blood Vessel Growth Thin-section both surgical and contralateral ear Count blood vessels under 400X magnification Data Analysis Define a statistical index to account for effect of sham surgery number of vessels present before surgery Neovascularization Index (treatment-CL) = average # of additional blood vessels in the implanted ear of treatment group (sham-CL) = average # of additional vessels due to the surgical procedure itself mean CL = average vessel count in all contralateral ears: effectiveness measure Results Conclusion Currently, data collection is still ongoing When calculated, compare NI of experimental case to NIs of control cases At this point, no definite conclusion can be drawn about the effect of Ang-1 and VEGF delivered through HA hydro