theroleofhumanxanthineoxidoreductase(hxor),anti-.docVIP

The role of Human xanthine oxidoreductase (HXOR), anti-HXOR antibodies and microorganisms in synovial fluid of patients with joint inflammation Najah Al- Muhtaseb 1* , Elham Al-Kaissi 2*, Abdul Jalil Thawaini 3, Zuhair Muhi eldeen 1 , Sabah Al- Muhtaseb 4, Badiee Al-Saleh5 1Department of Medicinal chemistry and Pharmacognosy, Faculty of Pharmacy, Petra University, Amman, Jordan 2Department of Pharmaceutics and biotechnology, Faculty of Pharmacy, Petra University, Amman, Jordan 3Department of Basic sciences, Faculty of Pharmacy, Philadelphia University, Amman, Jordan 4Department of Allied Medical Sciences, Faculty of Al-Balqa , University of Zarqa, Zarqa, Jordan 5Outpatient Clinic, Rheumatologist, Amman, Jordan P.O. Box 961343 Amman, Jordan, Fax 00962-6-5715551, Tel. 00962-6 E mails: 1naj_nim@ 2 Kaielham@ 3 ajthaini@ 1b Eldeenz@ 4sabahesa@ 5 badie_saleh@ * Contributed equally Abstract Objectives: This work is to investigate the levels of human xanthine oxidoreductase (HXOR), its antibodies and microorganisms in synovial fluid of patients with untreated rheumatoid joint diseases and to assess a possible relationship between severity of the inflammatory condition and the level of anti-XOR titers. Methods: Synovial fluids were collected from sixty four patients with rheumatoid joint diseases who had not been treated with corticosteroid or disease modifying antirheumatic drugs prior to study. Sixty four age matched individuals were included as control. XOR proteins level and anti XOR antibodies were determined in the blood and synovial fluid, using human XOR as antigen, by enzyme linked immunosorbent assay (ELISA technique). C-reactive protein, ESR and rheumatoid factors were measured in patients blood, and were culture for bacteria and fungi. Results: The titers of XOR protein in the synovial fluid of patients with rheumatoid arthritis were 90.43 ± 23.37 μg/ml (mean ± SD, n=29) and up to 62.42 ± 8.74 μg/ml (mean ± SD, n=35) in other joint in