纳米羟基磷灰石封闭牙本质小管扫描电镜观察.pdfVIP

中国医药 2015 年 11 月第 10 卷第 11 期 China Medici肘， November2015 , Vol. 10 , No.11 纳米连基磷灰石封闭牙本质小管 扫描电镜观察 于清华刘红艳刘志国 [摘要] 目的 观察纳米远基磷灰石对牙本质小管的封闭效果。方法 收集新鲜拔除的完整、无踊 的第三磨牙，制备牙本质片，完全随机分为人工唾液对照组、纳米在基磷灰石组、 Gluma 脱敏剂组，每组 18 片。分别采用人工唾液、纳米起基磷灰石和 Gluma 脱敏剂处理 7 d.然后模仿日常刷牙方法用去离子水 涂刷标本，持续 7 d 后扫描电镜观察牙本质片横切面牙本质小管封闭效果和纵切面渗透深度。结果 与人 工唾液对照组相比，横切面纳米挂基磷灰石堵塞部分牙本质小管口，堵塞物与管壁之间可见缝隙， Gluma 脱 敏剂在牙本质小管口表面形成封闭层，纵切面纳米在基磷灰石粉体部分渗入到牙本质小管内，渗入深度深 浅不一， Gluma 脱敏剂未见渗入。结论 纳米资基磷灰石可有效地封闭牙本质小管。 [关键词] 牙本质过敏症; 纳米起基磷灰石; 牙本质小管; 扫描电镜 [中图分类号] R 78 1. 2 [文献标识码 1 A Effect of nano-hydroxyapatite on dentinal tubule occlusion evaluated by scanning electron microscopy Yu Qingh圳 ， Liu Hongyan ， μu Zhigω. Department 0/ Endodontics , Guanghua School 0/ Stomatology , Ho机talo/ Stomotology , Sun Y.αt-sen University , Guangdong Pr，即incial Key Laborat01γ0/ Stomotology , Guangdong Province , Guangzhou 510055 , China Corresponding author: Yu Qinghua , Email: yuqinghua1966@126.com [ Abstract 1 Objective To observe the effecl of nano-h均E哼ydro芷勾ya叩pa挝甜tit忧e (nHA) 0仙n de臼nti by scanning electron microscopy (SEM)卜. Methods The third molars extracted from healthy adults were collected and prepared into slices. The specimen were randomly divided into artificial saliva control group , nHA group and Gluma desensitizer group. After 7 days treatment by artificial saliva , nHA and Gluma desensitizer , the dentine discs were brushed by micro-applicator with deionized water for 2 minutes , twice a day for extra 7 days. Occlusion of dentinal tubules in dentin discs were observed by SEM and the penetrating depth were compared among groups. Results Compared with artificial saliva , nHA crystals partially occluded dentinal tubules and penetrated into the tubul白; gluma desensitizer formed a sealing layer on partially dentin surface , but no dentin permeability was observed. Conclusion nHA can occlude the dentinal tubules effectively. [Key words 1 Dentin hypersensitivity; Nano句droxyapatite; Dentinal tubules; Scanning electron mlcroscopy 米瓷基磷