Wang_Narsimhan_2005_Interfacial Dilatational Elasticity and Viscosity of β-Lactoglobulin at.pdfVIP
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Interfacial Dilatational Elasticity and Viscosity of
a-Lactoglobulin at Air-Water Interface Using Pulsating
Bubble Tensiometry
Zebin Wang and Ganesan Narsimhan*
Biochemical and Food Process Engineering, Department of Agricultural and Biological
Engineering, Purdue University, West Lafayette, Indiana 47907-2093
Received October 25, 2004. In Final Form: February 3, 2005
The ability of proteins to provide stability in foams is greatly influenced by their interfacial dilatational
rheologicalproperties.Surface tensionresponseofapulsatingbubblewithanadsorbed layerofa-lactoglobulin
was measured for different frequencies and protein concentrations using a pulsating bubble tensiometer.
A methodology, accounting for adsorption/desorption as well as variation of surface concentration due to
expansion/contraction, was developed for the evaluation of surface dilatational elasticity and viscosity at
different frequencies from these measurements. The adsorption rate constants were inferred from the
surface pressure dynamics of protein adsorption using a Langmuir minitrough. The desorption rates were
shown to be negligible for a-lactoglobulin from the surface pressure response of a spread monolayer when
subjected to compression in a Langmuir minitrough. The proposed model was employed to infer the interfacial
dilatational viscosity and elasticity of an adsorbed a-lactoglobulin layer at the air-water interface from
experimental pulsating bubble data for protein concentrations in the range of 0.01-0.5 wt % at pH 7. As
expected, the interfacial dilatational rheological properties were found to be higher at higher protein
concentrations, this effect being less pronounced for dilatational elasticity. Heating at 80 °C for 30 min
was found to result in higher interfacial dilatational viscosity and lower interfacial dilatational elasticity
though this difference was within experimental error. The traditional approach for the inference of interfacial
dilatational rheological properties is f
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