中山大学考研《生物化学》07生物化学试题-2007秋季，A卷.docVIP

生物化学I（200年秋季学期, A卷） 专业(班级)： 姓名： 学号： 任课教师： 题号 合计 分数 《中山大学授予学士学位工作细则》第六条：“考试作弊不授予学士学位”。 I.（共小题，） ?????????????糖苷键连接；纤维素由葡萄糖通过?????????????糖苷键连接. 寡糖与蛋白的O连接指 ，N连接指 ； 指示某段DNA处于功能活动状态的特殊结构有Z-DNA、 、 等. 在Sanger法DNA自动测序中，荧光基团可以标记在 上或标记在 上. 结构性脂类包括磷脂、糖脂和?????????????，有些磷脂、糖脂的骨架不是甘油而是 ，因此又被称为鞘脂 II. 简答题（小题，）? 物质跨膜运输的类型有那些？A protein of unknown structure has been purified and is subjected to gel filtration chromatography under different conditions, with the following results: 1) Chromatography under native conditions suggests a molecular weight of 240,000 from comparison to standards of known molecular weight chromatographed on the same column; 2) Chromatography in the presence of 5 M urea yields a single protein peak corresponding to a M.W. of 60,000 from comparison to standards of known molecular weight chromatographed on the same column in urea. 1. What information do the results of (1) and (2) give about the structure of the protein? Describe the structure as completely as the data permit; 2. On an SDS polyacrylamide gel, the pure protein shows two stained bands. The relative mobilities of those 2 unknown bands are shown on the plot below as open circles (O), with the mobilities of 5 known polypeptide standards of M.W. 15,000; 20,000; 30,000, 40,000 and 50,000. Given the results of the SDS gel, how would you alter your description of the protein structure from the description you gave in part 1? Be as specific as you can about your revised description of the structure, and explain the reason for the altered description. IV. Lysozyme destroys the cell wall of bacteria by catalyzing the hydrolysis of 1,4-β-linkages between N-acetylmuramic acid (NAM) and N-acetyl-D-glucosamine (NAG) residues in a peptidoglycan. Six hexoses of the substrate fit into the active site of lysozyme, a deep cleft across part of the globular protein surface this cleft. Figure A shows the hydrolytic process of