银屑病患者角质形成细胞凋亡以及维A酸与榄香烯的影响x.docxVIP

网址： HYPERLINK  第  PAGE 5 页，共  NUMPAGES 5 页 银屑病患者角质形成细胞凋亡以及维A酸与榄香烯的影响 银屑病患者角质形成细胞凋亡以及维A酸与榄香烯的影响 中华皮肤科杂志 1999年第2期第32卷 论著 作者：刘振祥　李世荫 单位：100083 北京医科大学第三医院皮肤科 关键词：银屑病；细胞死亡；维甲酸；榄香烯 【摘　要】　目的　探讨银屑病患者角质形成细胞凋亡以及维A酸与榄香烯对体外培养的角质形成细胞凋亡的影响。方法　用TUNEL法测定28例银屑病患者角质形成细胞凋亡；流式细胞仪和DNA片段化观察维A酸与榄香烯对体外培养的角质形成细胞凋亡的影响。结果　28例银屑病患者中19例有大量凋亡细胞分布于表皮各层，另9例较少，分布于棘细胞层和颗粒层。浓度为1、5、10、20μg/mL的维A酸和浓度为20、40、60、80μg/mL的榄香烯可促进人角质形成细胞株Colo-16细胞凋亡。结论　银屑病患者角质形成细胞凋亡异常增多，一定浓度的维A酸与榄香烯可在实验室内促进人角质形成细胞株Colo-16细胞凋亡。 Apoptosis of Psoriatic Keratinocytes and the Effects of Retinoic Acid and Elemene on Apoptosis of Cultured Keratinocytes LIU Zhenxiang, LI Shiyin. Department of Dermatology, Third Hospital of Beijing Medical University, Beijing 100083 【Abstract】　Objective　To investigate apoptosis of psoriatic keratinocytes and the effects of retinoic acid and elemene on apoptosis of cultured keratinocytes.Methods　Apoptosis of psoriatic keratinocytes and the effects of retinoic acid and elemene on apoptosis of cultured keratinocytes were studied with TUNEL method, flow cytometry and DNA fragmentation extraction.Results　It was found that there were a large number of apoptotic cells throughout all layers of psoriatic epidermis in 19 of 28 patients and relatively small amount in upper epidermal layers in 9 of 28 patients. Retinoic acid at concentrations of 1,5,10,20μg/mL and elemene at 20,40,60,80μg/mL could induce apoptosis of cultured Colo-16 keratinocytes in vitro.Conclusion　These results indicate that the number of apoptotic cells increased abnormally in psoriasis. Retinoic acid and elemene at certain concentrations could induce apoptosis of cultured Colo-16 keratinocytes in vitro. 【Key words】　PsoriasisApoptosisTretinoinElemene 近年来人们认识到细胞凋亡不仅在皮肤发育、更新、平衡稳定中起作用，在某些增殖性皮肤病如银屑病中也表现异常。许多细胞的增殖都需要双重信号，第一信号有引起细胞增殖或凋亡两种可能性，第二信号决定细胞进入增殖状态，若没有第二信号的作用，细胞进入凋亡状态。因此，细胞增殖与凋亡密切相关。我们从细胞凋亡的角度探讨银屑病患者角质形成细胞增殖过度的机理，采用TUNEL法观察银屑病患者角质形成细胞凋亡的变化，同时应用流式细胞仪和DNA片段化分析观察不同浓度的维A