131I-Rituximab for B-cell lymphoma Experimental Study of biological effects.docVIP

 PAGE \* MERGEFORMAT 10 131I-Rituximab for B-cell lymphoma Experimental Study of biological effects Author: Cai Qiuqiong, Du Hua, Bao-An Chen, Zhong, Huang Ke, Qu Hai Boat 【Abstract】 Objective: To study the 131I labeled Rituximab for B-cell lymphoma as well as the biological effects of xenografts in nude mice bearing human Raji cell immune therapeutic effects of radiation for radioimmunotherapy provide experimental evidence. Methods: In vitro cultured human B-cell lymphoma cell line Raji cells and Raji cells in nude mice were inoculated subcutaneously into the tumor, IODO-GEN method will 131I marked on the Rituximab, cells were divided into 131I-Rituximab group, pure antibody group, simple group of radionuclides and blank control group, four groups, each group detected by flow cytometry Raji cell apoptosis and cell cycle; take 30 into nude mice were randomly divided into high, medium and low-dose treatment group and the pure antibody group, simple radionuclide group, blank control group, 6 groups to conduct research radioimmunotherapy in nude mice. Weekly measurement of tumor size in nude mice 1, nude mice were sacrificed after 4 weeks, take tumor weighing, routine pathological analysis. Results: 131I-Rituximab group of apoptosis rate was higher than the other groups; 131I-Rituximab group of cell cycle changes, most of arrest in G2 phase. Nude mice each treatment group and control group, tumor growth slowed down tumor growth inhibition rate in a dose-time-dependent histopathological test of the treatment effective. Conclusion: 131I-Rituximab could induce Raji cell apoptosis and regulation of cell cycle and can be specifically targeted at the tumor tissue, play a role in radioimmunotherapy has potential clinical application. Keywords: radioimmunotherapy; B-cell lymphoma; anti-CD20 monoclonal antibody; 131I-Rituximab Non-Hodgkin’s lymphoma (NHL) is the most common malignant tumor of the lymphatic system, which the vast majority of B-cell